Characterization and genomic Analysis of a novel Pseudomonas phage vB_PsaP_M1, representing a new viral family, Psaeviridae

Pseudomonas is a ubiquitous and ambiguous opportunistic pathogen, plays an important ecological role in the ocean. Here, new species, phage vB_PsaP_M1, described, which was isolated from surface coastal waters of Qingdao, China. vB_PsaP_M1 contains linear, double-stranded 89,387-bp genome with GC content 41.04% encoding 184 putative open reading frames (ORFs). There were 50 conservative domains predicted BLASTp, including two auxiliary metabolic genes (Phosphate-inducible gene phoH signal peptide peptidase A, sppA ). Phylogenetic analysis whole amino acid sequence comparative genomic showed that has distant evolutionary relationship previously viruses can be grouped into family-level novel viral cluster (VC_61) eleven uncultured, assembled genomes, named as Psaeviridae. Psaeviridae ORFs (ORFs 117 127), not detected genomes other families, confirming proposal for family. Combined its ability to infect representation unstudied family, may model system study interactions between host cells marine ecosystems.